Journal: EBioMedicine
Article Title: CD24-targeted intraoperative fluorescence image-guided surgery leads to improved cytoreduction of ovarian cancer in a preclinical orthotopic surgical model.
doi: 10.1016/j.ebiom.2020.102783
Figure Lengend Snippet: Fig. 1. CD24 is highly expressed in ovarian cancer cell lines and primary ovarian cancer cells, and can be detected by CD24-AF750 NIR staining. (a) Flow cytometry analysis of EOC cell lines OV-90, Caov-3, COV318 and Skov-3 stained with the monoclonal antibody CD24 conjugated to AF750 (n = 3). Each column represents the mean with SD. (b) CD24 expres- sion was evaluated by flow cytometry, and antigen-binding capacity was quantified for OV-90 compared to monoclonal IgG1 antibody (mAb) control. (c) Validation of target-specific binding. White light (colour) image, near infrared (NIR 800) fluorescent image and pseudo-coloured fluorescence intensity merge image of CD24-AF750 stained EOC cell pellets (25 £ 107 tumour cells, scale bar = 1 cm). (d) Immunohistochemistry of four different patient-derived xenograft (PDX) ovarian tumours, stained with anti-CD24. Immunoreactivity score (IRS) 425 § 096 (scale: 1ow = 1 7 = high). Images were captured at 100£ and 200£ magnification (scale bar = 25 mm).
Article Snippet: An unconjugated monoclonal CD33 IgG1 antibody (Clone WM53, Cat# MCA1271T, RRID: AB_2074234, Bio-Rad) was used as a negative control.
Techniques: Staining, Flow Cytometry, Cytometry, Binding Assay, Control, Biomarker Discovery, Immunohistochemistry, Derivative Assay